Freshly isolated growth cells obtained from unfixed eye specimens (n = 7) were analyzed in support of the presence of CD44, ABCG2, CXCR4, CD133, and CD90 using surge cytometry. RT-PCR was performed to examine the air of human being Syntaxin1A, PROX1, CD133, and NSE in the sorted subpopulation of growth cells.
Results. Two altered subpopulations of cells were practical in seven samples. The small cells, assigned FSClo/SSClo (forward scatter low/side scatter low, ranging from 1.7% to 17.7%) were characterized as activist in support of CD44 and pessimistic in support of CD133, CXCR4, and CD90. The copious cells were designated as FSChi/SSClo (ranging from 2.7% to 35.1%) and characterized as activist in support of all markers. RT-PCR analysis revealed with the purpose of sorted cells of FSClo/SSClo subpopulation uttered the retinal progenitor cell markers PROX1 and Syntaxin1A.